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How do you test for BRAF?
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How do you test for BRAF?

› Forums › General Melanoma Community › How do you test for BRAF?

  • Post
    • June 15, 2011 at 4:58 pm
    Hope Returning
    Participant

      Is the BRAF test a simple blood test? How do you test for it/?

      Is the BRAF test a simple blood test? How do you test for it/?

    Viewing 3 reply threads
    • Replies
        • June 15, 2011 at 5:09 pm
        shellebrownies
        Participant

          BRAF test has to come from a melanoma tumor tissue sample. I believe they have to isolate DNA from the tumor and then look for the BRAF genetic mutation. Usually takes 2-3 weeks to get the result, assuming they got a good, viable sample. 

          In our case, my husband's original tumor sample didn't have have enough of what they need to process the BRAF. Luckily, since they had to take a sample from a liver lesion to verify his staging, they took another sample then. He did end up testing positive for this mutation.

          If you have any measurable disease, I would highly recommend getting this test done. It's good information to have and can save you time later on if you need to choose medical trials.

          Michelle, wife of Don

            • June 15, 2011 at 7:25 pm
            Hope Returning
            Participant

              Thanks a lot for the information. It will be very usefull. Thanks a lot.

              • June 15, 2011 at 7:59 pm
              MichaelFL
              Participant

                Make it clear you want to be tested for the BRAF V600E mutation.

                There is also others such as V600K, V600D, V600R and more. Believe it or not, the wrong one has been done and time was wasted.

                Michael

                • June 15, 2011 at 7:59 pm
                MichaelFL
                Participant

                  Make it clear you want to be tested for the BRAF V600E mutation.

                  There is also others such as V600K, V600D, V600R and more. Believe it or not, the wrong one has been done and time was wasted.

                  Michael

                  • June 16, 2011 at 1:04 am
                  shellebrownies
                  Participant

                    Hey, Michael, I did not know that! What are the other ones for, do you know?

                    • June 16, 2011 at 1:04 am
                    shellebrownies
                    Participant

                      Hey, Michael, I did not know that! What are the other ones for, do you know?

                      • June 16, 2011 at 1:40 am
                      MichaelFL
                      Participant

                         

                        I hope this does not confuse you, but here it is in a nutshell:

                        In relation to BRAF: Codons 595-600 of Exon 15 of the BRAF proto-oncogene are usually checked as it is the most common area for the mutation, and #600 is usually called the “hot spot” as that is where they mostly occur. (In the study below, exon 11 was also tested)

                        In relation to NRAS: Exons 2 and 3 may be checked, and Codons 12, 13 and 61, (61 is also most common area) are usually tested.

                        Here are some of the more common ones. Out of 90 melanoma samples that were analyzed in a study, 85 came back with results. It also included NRAS and KIT.

                        BRAF—Exon 15:

                        V600E 60 of 90 (amount of 90 positive)

                        V600K 5 of 90

                        V600R 1 of 90

                        BRAF—Exon 11:

                        G469A 1 of 90

                        NRAS—Exon 3:

                        Q61K 5 of 90

                        Q61L 2 of 90

                        Q61R 5 of 90

                        NRAS—Exon 2:

                        G13R 2 of 90

                        G13D 2 of 90

                        KIT:

                        G565V/N822I 1 of 90

                        P577L 1 of 90

                        I have a even bigger list, that includes other cancers, but I do not want to confuse anyone further, so I will leave it out.

                        Michael

                        • June 16, 2011 at 1:48 am
                        MichaelFL
                        Participant

                          Keep in mind that concerning BRAF, the majority of the clinical trials are done with the V600E mutation being positive. I believe there are also a few with the V600K mutation as well. Without checking clinicaltrials.gov, I am not sure about the V600R or the rest, but as far as I remember, I do not think there are any trials for the other mutations listed.

                          Michael

                          • June 16, 2011 at 7:01 am
                          JerryfromFauq
                          Participant

                            I believe the PDGF PLX4032 only takes the V600E-BRAF mutational tumors._

                            At one time  the  GSK trials took any positive BRAF mutation.  If ne is beiing tested for the PlX4032 trial they will be told that they are BRAF negative even if they have any of the other BrAF proteiins/Mutations.  One must understand exactly what tst is really being run.and what the terms positive and negative mean for that particular test..

                            • June 16, 2011 at 7:46 am
                            JerryfromFauq
                            Participant

                              OOP's wrong info in clipboard.   I believe the Plexion/Roche PLX4032 only takes the V600E-BRAF mutational tumors

                              • June 16, 2011 at 7:46 am
                              JerryfromFauq
                              Participant

                                OOP's wrong info in clipboard.   I believe the Plexion/Roche PLX4032 only takes the V600E-BRAF mutational tumors

                                • June 16, 2011 at 7:01 am
                                JerryfromFauq
                                Participant

                                  I believe the PDGF PLX4032 only takes the V600E-BRAF mutational tumors._

                                  At one time  the  GSK trials took any positive BRAF mutation.  If ne is beiing tested for the PlX4032 trial they will be told that they are BRAF negative even if they have any of the other BrAF proteiins/Mutations.  One must understand exactly what tst is really being run.and what the terms positive and negative mean for that particular test..

                                  • June 16, 2011 at 7:12 am
                                  JerryfromFauq
                                  Participant

                                    Thanks for the mutational listing.  I like it.  Send me yoour URL reference, please.  Thanks, Jerry

                                    • June 16, 2011 at 7:12 am
                                    JerryfromFauq
                                    Participant

                                      Thanks for the mutational listing.  I like it.  Send me yoour URL reference, please.  Thanks, Jerry

                                      • June 16, 2011 at 1:48 am
                                      MichaelFL
                                      Participant

                                        Keep in mind that concerning BRAF, the majority of the clinical trials are done with the V600E mutation being positive. I believe there are also a few with the V600K mutation as well. Without checking clinicaltrials.gov, I am not sure about the V600R or the rest, but as far as I remember, I do not think there are any trials for the other mutations listed.

                                        Michael

                                        • June 16, 2011 at 1:40 am
                                        MichaelFL
                                        Participant

                                           

                                          I hope this does not confuse you, but here it is in a nutshell:

                                          In relation to BRAF: Codons 595-600 of Exon 15 of the BRAF proto-oncogene are usually checked as it is the most common area for the mutation, and #600 is usually called the “hot spot” as that is where they mostly occur. (In the study below, exon 11 was also tested)

                                          In relation to NRAS: Exons 2 and 3 may be checked, and Codons 12, 13 and 61, (61 is also most common area) are usually tested.

                                          Here are some of the more common ones. Out of 90 melanoma samples that were analyzed in a study, 85 came back with results. It also included NRAS and KIT.

                                          BRAF—Exon 15:

                                          V600E 60 of 90 (amount of 90 positive)

                                          V600K 5 of 90

                                          V600R 1 of 90

                                          BRAF—Exon 11:

                                          G469A 1 of 90

                                          NRAS—Exon 3:

                                          Q61K 5 of 90

                                          Q61L 2 of 90

                                          Q61R 5 of 90

                                          NRAS—Exon 2:

                                          G13R 2 of 90

                                          G13D 2 of 90

                                          KIT:

                                          G565V/N822I 1 of 90

                                          P577L 1 of 90

                                          I have a even bigger list, that includes other cancers, but I do not want to confuse anyone further, so I will leave it out.

                                          Michael

                                          • June 15, 2011 at 7:25 pm
                                          Hope Returning
                                          Participant

                                            Thanks a lot for the information. It will be very usefull. Thanks a lot.

                                          • June 15, 2011 at 5:09 pm
                                          shellebrownies
                                          Participant

                                            BRAF test has to come from a melanoma tumor tissue sample. I believe they have to isolate DNA from the tumor and then look for the BRAF genetic mutation. Usually takes 2-3 weeks to get the result, assuming they got a good, viable sample. 

                                            In our case, my husband's original tumor sample didn't have have enough of what they need to process the BRAF. Luckily, since they had to take a sample from a liver lesion to verify his staging, they took another sample then. He did end up testing positive for this mutation.

                                            If you have any measurable disease, I would highly recommend getting this test done. It's good information to have and can save you time later on if you need to choose medical trials.

                                            Michelle, wife of Don

                                            • June 16, 2011 at 8:28 pm
                                            Tim–MRF
                                            Guest

                                              Lot's of great information from other posts.  I will just add one note.  In order to get the Roche/Genentech drug, called PLX4032 or vemurafenib, your tumor must test positive on the test that Roche makes.  Even if you have a positive test for BRAF V600e mutation from a certified lab using a certified kit, you cannot get into a clinical trial or the expanded access (compassionate use) program unless you test positive with the Roche kit.

                                              Once the FDA approves the drug that requirement may go away.  The "label" may simply specify a positive V600e test by a certified lab.

                                              And you may all know this, but the name "vemurafenib" was derived from the "v" and "e" in V600e, then "mu" for "mutation" and "raf" from BRAF.  Amazing that someone actually thinks of this stuff.

                                               

                                              Tim–MRF

                                                • June 22, 2011 at 2:15 pm
                                                dian in spokane
                                                Participant

                                                  Thanks Tim and thanks Michael for all the info. I received a note from the Cell Biology lab down in california yesterday telling me they had some of my tumor cell samples and would like permission to dispose of it. I was thinking I might try to see if that could be tested for BRAF mutation, but had some memory that the actual trial would require a new test, so your info is timely.

                                                  I also wondered how they came up with that name. What about 'Yervoy'? how'd they get that? very interesting.

                                                  dian

                                                  • June 22, 2011 at 2:15 pm
                                                  dian in spokane
                                                  Participant

                                                    Thanks Tim and thanks Michael for all the info. I received a note from the Cell Biology lab down in california yesterday telling me they had some of my tumor cell samples and would like permission to dispose of it. I was thinking I might try to see if that could be tested for BRAF mutation, but had some memory that the actual trial would require a new test, so your info is timely.

                                                    I also wondered how they came up with that name. What about 'Yervoy'? how'd they get that? very interesting.

                                                    dian

                                                  • June 16, 2011 at 8:28 pm
                                                  Tim–MRF
                                                  Guest

                                                    Lot's of great information from other posts.  I will just add one note.  In order to get the Roche/Genentech drug, called PLX4032 or vemurafenib, your tumor must test positive on the test that Roche makes.  Even if you have a positive test for BRAF V600e mutation from a certified lab using a certified kit, you cannot get into a clinical trial or the expanded access (compassionate use) program unless you test positive with the Roche kit.

                                                    Once the FDA approves the drug that requirement may go away.  The "label" may simply specify a positive V600e test by a certified lab.

                                                    And you may all know this, but the name "vemurafenib" was derived from the "v" and "e" in V600e, then "mu" for "mutation" and "raf" from BRAF.  Amazing that someone actually thinks of this stuff.

                                                     

                                                    Tim–MRF

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