genetic and molecular types of melanoma?

Hi Hazel,

My husband had the 50-Gene Somatic Mutation Analysis Panel done on his tumor.  If you remember, he has a similar case where they can't rule out metastatic melanoma since there is no intraepidermal component (some have said primary dermal melanoma but our oncologist at MD Anderson won't say that). He had 19 mm melanoma removed from back but scans to date have shown NED and he is not doing any treatments.    He was found to have NRAS mutation in his.  Here is an image from the genetic mutation test from MD Anderson.  Looks like BRAF, KIT, and NRAS are what they look for in Melanoma (I beleive NRAS is found less frequently than BRAF):

50-Gene Somatic Mutation Analysis Panel Report**:

Clinical test requisition for mutation studies on the following genes was received: BRAF, KIT, NRAS

A next generation sequencing (NGS)-based analysis for the detection of somatic mutations in the coding sequence of a total of 50 genes
was performed on the DNA extracted from the sample in our CLIA-certified molecular diagnostics laboratory. Interpretative findings are
reported in the mutation screening summary table(s) below followed by specific details of detected variants.

Interpretation Key:

Circled/Bold: Mutation (or variant) detected
Underlined: Mutation testing requested (ordered gene)
Asterisk: Additional confirmation studies in progress, addendum report will be issued

MUTATION SCREENING SUMMARY:

ABL1 CSF1R FGFR2 IDH1 MLH1 PTPN11 TP53
AKT1 CTNNB1 FGFR3 IDH2 MPL RB1 VHL
ALK EGFR FLT3 JAK2 NOTCH1 RET
APC ERBB2 GNA11 JAK3 NPM1 SMAD4
ATM ERBB4 GNAQ KDR NRAS SMARCB1
BRAF EZH2 GNAS KIT PDGFRA SMO
CDH1 FBXW7 HNF1A KRAS PIK3CA SRC
CDKN2A FGFR1 HRAS MET PTEN STK11

I. Mutations in ordered genes
Gene Standardized Nomenclature (HGVS) Location DNA change Protein change dbSNP ID COSMIC
ID
NRAS NM_002524.4(NRAS):c.181C>A p.Q61K Exon 3 SNV Missense rs121913254COSM580

I have been trying for a long time to get an answer to this. Wild type mutations make up about 28% of the mutations. Among that 28%, are the wild types a singular muations, or are they a combination of "none of the above"?

Can anyone clarify this.

Thanks.

I have been trying for a long time to get an answer to this. Wild type mutations make up about 28% of the mutations. Among that 28%, are the wild types a singular muations, or are they a combination of "none of the above"?

Can anyone clarify this.

Thanks.

I have been trying for a long time to get an answer to this. Wild type mutations make up about 28% of the mutations. Among that 28%, are the wild types a singular muations, or are they a combination of "none of the above"?

Can anyone clarify this.

Thanks.

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi,  Thank you for posting.  I have st 3 Mel BRAF600+.  How does the information you've received on this panel inform the treatment options which you may be given?  How else might the information be useful?  Thank you, Ann

Hi Hazel,

My husband had the 50-Gene Somatic Mutation Analysis Panel done on his tumor.  If you remember, he has a similar case where they can't rule out metastatic melanoma since there is no intraepidermal component (some have said primary dermal melanoma but our oncologist at MD Anderson won't say that). He had 19 mm melanoma removed from back but scans to date have shown NED and he is not doing any treatments.    He was found to have NRAS mutation in his.  Here is an image from the genetic mutation test from MD Anderson.  Looks like BRAF, KIT, and NRAS are what they look for in Melanoma (I beleive NRAS is found less frequently than BRAF):

50-Gene Somatic Mutation Analysis Panel Report**:

Clinical test requisition for mutation studies on the following genes was received: BRAF, KIT, NRAS

A next generation sequencing (NGS)-based analysis for the detection of somatic mutations in the coding sequence of a total of 50 genes
was performed on the DNA extracted from the sample in our CLIA-certified molecular diagnostics laboratory. Interpretative findings are
reported in the mutation screening summary table(s) below followed by specific details of detected variants.

Interpretation Key:

Circled/Bold: Mutation (or variant) detected
Underlined: Mutation testing requested (ordered gene)
Asterisk: Additional confirmation studies in progress, addendum report will be issued

MUTATION SCREENING SUMMARY:

ABL1 CSF1R FGFR2 IDH1 MLH1 PTPN11 TP53
AKT1 CTNNB1 FGFR3 IDH2 MPL RB1 VHL
ALK EGFR FLT3 JAK2 NOTCH1 RET
APC ERBB2 GNA11 JAK3 NPM1 SMAD4
ATM ERBB4 GNAQ KDR NRAS SMARCB1
BRAF EZH2 GNAS KIT PDGFRA SMO
CDH1 FBXW7 HNF1A KRAS PIK3CA SRC
CDKN2A FGFR1 HRAS MET PTEN STK11

I. Mutations in ordered genes
Gene Standardized Nomenclature (HGVS) Location DNA change Protein change dbSNP ID COSMIC
ID
NRAS NM_002524.4(NRAS):c.181C>A p.Q61K Exon 3 SNV Missense rs121913254COSM580

Hi Hazel,

My husband had the 50-Gene Somatic Mutation Analysis Panel done on his tumor.  If you remember, he has a similar case where they can't rule out metastatic melanoma since there is no intraepidermal component (some have said primary dermal melanoma but our oncologist at MD Anderson won't say that). He had 19 mm melanoma removed from back but scans to date have shown NED and he is not doing any treatments.    He was found to have NRAS mutation in his.  Here is an image from the genetic mutation test from MD Anderson.  Looks like BRAF, KIT, and NRAS are what they look for in Melanoma (I beleive NRAS is found less frequently than BRAF):

50-Gene Somatic Mutation Analysis Panel Report**:

Clinical test requisition for mutation studies on the following genes was received: BRAF, KIT, NRAS

A next generation sequencing (NGS)-based analysis for the detection of somatic mutations in the coding sequence of a total of 50 genes
was performed on the DNA extracted from the sample in our CLIA-certified molecular diagnostics laboratory. Interpretative findings are
reported in the mutation screening summary table(s) below followed by specific details of detected variants.

Interpretation Key:

Circled/Bold: Mutation (or variant) detected
Underlined: Mutation testing requested (ordered gene)
Asterisk: Additional confirmation studies in progress, addendum report will be issued

MUTATION SCREENING SUMMARY:

ABL1 CSF1R FGFR2 IDH1 MLH1 PTPN11 TP53
AKT1 CTNNB1 FGFR3 IDH2 MPL RB1 VHL
ALK EGFR FLT3 JAK2 NOTCH1 RET
APC ERBB2 GNA11 JAK3 NPM1 SMAD4
ATM ERBB4 GNAQ KDR NRAS SMARCB1
BRAF EZH2 GNAS KIT PDGFRA SMO
CDH1 FBXW7 HNF1A KRAS PIK3CA SRC
CDKN2A FGFR1 HRAS MET PTEN STK11

I. Mutations in ordered genes
Gene Standardized Nomenclature (HGVS) Location DNA change Protein change dbSNP ID COSMIC
ID
NRAS NM_002524.4(NRAS):c.181C>A p.Q61K Exon 3 SNV Missense rs121913254COSM580